Application of Compact Surface Plasmon Resonance Biosensor on Antimicrobial Susceptibility test

ChihHan Chen^1*, Hsuan-Chen Lin¹, How-Foo Chen¹

¹生醫光電所, 國立陽明大學, 台北市, Taiwan

* Presenter:ChihHan Chen, email:kazafq@gmail.com

The Surface Plasmon Resonance (SPR) technique has been utilized in biosensing for decades. Due to its characteristics of label-free, high-sensitive and real-time observation, SPR biosensors are widely used in drugs development and life science research. Here we introduce a home-made compact SPR device using a disposable polymer-molding prism with the two 1D parabolic side surfaces and targeted at satisfaction of the clinical need. By applying such a prism in a SPR system, we removed complicated mechanical components of a usual SPR biosensor and made the device more suitable for clinical usage. As a result, the test device can be down sized to 20cm*20cm*5cm and remains high sensitivity which reach to 10^-6 refractive index.
As the application, the surface plasmon resonance (SPR) biosensor was used for an antibiotic susceptibility test. Antimicrobial susceptibility test of standard E. Coli and Staphylococcus aureus from clinical tissues were performed based on the change of the refractive index of the bacterium strains subject to antibiotics. Using the polymer-molding prisms shaped with parabolic surfaces on both sides as the optical couplers and testing chips, the compact SPR biosensor is proved feasible for clinical usage.

Keywords: Antimicrobial Susceptibility test , Surface Plasmon Resonance, Biosensor, Compact, medical device