Multi-focal plane holographic confocal fluorescent microscopy

Tso-Hua Wu^1,2*, Chou-Min Chia², Haw Hsiao³, J.Andrew Yeh¹, Yuan Luo²

¹Institude of NanoEngineering and MicroSystems, National Tsing Hua University, Hsinchu, Taiwan
²Institute of Medical Device and Imaging, National Taiwan University, Taipei, Taiwan
³Institute of Mechanical engineering, National Taiwan University, Taipei, Taiwan

* Presenter:Tso-Hua Wu, email:b023021041@gmail.com

Confocal microscopy has been widely applied to obtain optical images. However, scanning point by point is time-consuming for the confocal technique. Both lateral and axial scanning is necessary because the confocal system is usually used to construct the three-dimensional images. Here, a non-axial-scanning slit confocal microscopy is presented. The proposed system combines multiplexed volume holographic gratings (MVHGs) in the illumination part and corresponding confocal slit apertures.

Keywords: Multiplex holography, Volume gratings, Confocal microscopy, Three-dimensional microscopy