Multi-focal plane holographic confocal fluorescent microscopy
Tso-Hua Wu1,2*, Chou-Min Chia2, Haw Hsiao3, J.Andrew Yeh1, Yuan Luo2
1Institude of NanoEngineering and MicroSystems, National Tsing Hua University, Hsinchu, Taiwan
2Institute of Medical Device and Imaging, National Taiwan University, Taipei, Taiwan
3Institute of Mechanical engineering, National Taiwan University, Taipei, Taiwan
* Presenter:Tso-Hua Wu,
Confocal microscopy has been widely applied to obtain optical images. However, scanning point by point is time-consuming for the confocal technique. Both lateral and axial scanning is necessary because the confocal system is usually used to construct the three-dimensional images. Here, a non-axial-scanning slit confocal microscopy is presented. The proposed system combines multiplexed volume holographic gratings (MVHGs) in the illumination part and corresponding confocal slit apertures.

Keywords: Multiplex holography, Volume gratings, Confocal microscopy, Three-dimensional microscopy