Differential phase contrast volume holographic incoherent imaging system

Yu-Hsin Chia^1*, Yuan Luo^1,2,3

¹Institute of Medical Device and Imaging, National Taiwan University, Taipei, Taiwan
²Molecular Imaging Center, National Taiwan University, Taipei, Taiwan
³YongLin Institute of Health, National Taiwan University, Taipei, Taiwan

* Presenter:Yu-Hsin Chia, email:bb0222020@gmail.com

Many optical microscopy systems have been designed to obtain three-dimensional (3D) images for biomedical request. However, most of these systems require mechanical or electro-optic axial scanning mechanism to obtain multi-depth images. In order to overcome this limit, we present a differential phase contrast volume holographic microscopy (DPC-VHM) based on asymmetric illumination to simultaneously obtain multi-depth phase contrast images of a biological samples in one shot. The volume holographic gratings composed of PQ-PMMA photosensitive materials that can provide high spectral and angular Bragg selectivity. Experimental results demonstrate the imaging ability of the proposed DPC-VHM system to observe the bright field and differential phase contrast multi-plane imaging of the sample.

Keywords: Three-dimensional imaging, Incoherent, Holography, Microscopy